LOCUS       40924_23774       11355 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB725 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11355)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11355)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2097
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     terminator      2119..2371
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2405..2421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2453..2483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2498..2519)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2555..2807)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2845..4140)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61232.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(2845..4140)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4192..4371)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4877..4901)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5422..6210
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6459..7047
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7233..7373)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7717..7911)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7980..9050)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9482..10108)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggct agcatgactg gtggacagca aatgggtgct
     2101 tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     2161 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     2221 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     2281 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     2341 tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc aattcgtaat
     2401 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2461 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattag
     2521 gctgaattag gcgcgcctat ttctattaat tcccgatcta gtaacataga tgacaccgcg
     2581 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     2641 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     2701 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2761 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcgcc
     2821 tcgagtgact tctctgattg cagactattt ataccaacca gcaagaaagt gtcgaagtat
     2881 gaagcagaag gcgcaggcta gagcctggaa aacgagaaga tggatgcaaa gagacttttc
     2941 tgatttagga ccaaacaacc tcaagtaaac attgacgagt gtcccatctt ttgtgccggt
     3001 cagtaatccg gtagcaggat cataccttgg gagacgatgg cgcggacatt tcacaatccc
     3061 agcaagctga ggaagcgata atagaaggtt cagaacttga ggaagaaaga agatgaggag
     3121 ggtttcactg aagtgaccca gaataccaac aactgccata gtcattccgg cgaagactgt
     3181 gtaagtgtct ccaacaaaaa ctgaagaagg ataccaattg taagcaagca ttgccaagga
     3241 tgtggccatc agcggttgag taagaaatat cgagaaggca tgagcttggt gatactcggg
     3301 atcaacagat gctccgattt gcataacgtt gtgtatcagg atagcagcgg caataacaac
     3361 tgtttgaccg atttcaaggc cattcagacc agcgtggatg ttaatagagt ttgtacaaaa
     3421 gacagcaagt agccccatat ataacttata tatccttcct agattaagca cttccaagcc
     3481 aatataagca acaagaggtt ttggtataac aatagttgta tgaccagcat aagccatcaa
     3541 aagtggaagg gtagcaaaag atggcaggac aagttttact ctccaaggca catcaaggac
     3601 atcatctaca aatccaagca aaatcatgaa gcaaatagat gctagtgctg cattatactc
     3661 cacaagccaa ttagaatctt cagtgaagtt aaaatactga aatattatcg ccacgatcaa
     3721 aaagacaata ccaacaacaa tacccaatga ctctggcact ttaatatctc cttgaggcgt
     3781 gcctcttttg ttgatatcaa aaccaaacat gttacgtctc agaacgtatc tggcagtaac
     3841 aggaatcatt ttgagagtga cgaaaaaccc aaccaagcta agccccgcgt taattagaat
     3901 cgatcgcttg agttcattat caaccttgta gtgaaaacag agaaggtata gatagagcga
     3961 acagagaaga gtagagatca cgaagatcac tcctaatttc ggcggcgcga gacgaaattc
     4021 ctcaccggaa gcagaaaccg tctttcgggt catcttctgc tccgatggcg ctgagttggg
     4081 ttccgacggg tccggtttgt tgggaattga gatcgatgaa gctctcttac gagctgccat
     4141 atcttattgc cccccgggat cagatctgga ttgagagtga atatgagact ctaattggat
     4201 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     4261 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     4321 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     4381 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     4441 ctccgctcat gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     4501 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     4561 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     4621 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     4681 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     4741 cttgcgccct gagtgcttgc ggcagcgtga agctgatccg tcgagattaa tagaaataaa
     4801 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     4861 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     4921 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     4981 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     5041 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     5101 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     5161 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     5221 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     5281 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     5341 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     5401 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     5461 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     5521 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     5581 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     5641 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     5701 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     5761 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     5821 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     5881 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     5941 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     6001 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     6061 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     6121 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     6181 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     6241 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     6301 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     6361 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6601 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7141 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7201 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     7261 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     7321 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     7381 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7441 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7501 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     7561 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     7621 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     7681 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     7741 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     7801 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     7861 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     7921 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     7981 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     8041 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     8101 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     8161 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     8221 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     8281 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     8341 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     8401 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     8461 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     8521 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     8581 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
     8641 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
     8701 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
     8761 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
     8821 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
     8881 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
     8941 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
     9001 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
     9061 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
     9121 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
     9181 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
     9241 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
     9301 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
     9361 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
     9421 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
     9481 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
     9541 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
     9601 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
     9661 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
     9721 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
     9781 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
     9841 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
     9901 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
     9961 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    10021 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    10081 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    10141 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    10201 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    10261 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    10321 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    10381 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    10441 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    10501 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    10561 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    10621 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    10681 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    10741 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    10801 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    10861 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    10921 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    10981 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    11041 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    11101 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    11161 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    11221 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    11281 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    11341 cgaacggata aacct
//
